Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics

• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan-Thang Cao,
• Vy Tran-Anh and
• Hieu Vu Quang

Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17

• . Determination of iron content with the Prussian blue protocol The iron content was determined by measuring absorption of Prussian blue at 562 nm (Biotek ELX800, Agilent, USA). Briefly, the material was initially reduced for 10 min in 4% HCl. Then, 4% K3[Fe(CN)6] was added and the mixture was incubated for an

• % using the nanoprecipitation method [29]. The loading of iron oxide nanoparticles The entrapment of iron oxide NPs into PLGA nanoparticles was estimated by measuring the absorbance at 562 nm through the Prussian blue reaction. The entrapment of IO was approx. 1.11% of the total weight of nanoparticles

Nanoarchitectonics of photothermal materials to enhance the sensitivity of lateral flow assays

• Elangovan Sarathkumar,
• Rajasekharan S. Anjana and
• Ramapurath S. Jayasree

Beilstein J. Nanotechnol. 2023, 14, 988–1003, doi:10.3762/bjnano.14.82

• typhimurium bacteria using Prussian blue and a Au composite. The results proved that photothermal signal amplification yielded a much higher sensitivity (101 CFU·mL−1) than the colorimetric method (102 CFU·mL−1) [86]. Before that, the same group developed a MoS2@Au nanocomposite for the photothermal detection

• of the same Salmonella typhimurium bacterium with a LOD of 102 CFU·mL−1, which is lower than that of the Prussian blue–GNP composite [87]. From the above examples, it is clear that size, shape, and composition of a nanomaterial play significant roles in the photothermal properties and the efficiency

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

• Brunno R. F. Verçoza,
• Robson R. Bernardo,
• Luiz Augusto S. de Oliveira and
• Juliany C. F. Rodrigues

Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73

• promastigotes and intracellular amastigotes incubated with Prussian blue revealed that both parasite stages can uptake the SPIONs (Figure 1). The arrows and arrowheads in Figure 1 show the characteristic blue stain that indicates the positive reaction between potassium ferrocyanide and ferrous compounds. In

• standard model for cutaneous leishmaniasis. The parasites were maintained according to previously published protocols [22]. Prussian blue staining For staining with Prussian blue (Sigma-Aldrich, Germany), promastigote and intracellular amastigotes were treated with 100 µg/mL of SPIONs for 24 h. The

• (*). Bright-field optical microscopy of L. amazonensis promastigotes (A, B) and intracellular amastigotes (C, D) treated with 100 µg/mL of SPIONs for 24 h, after staining with Prussian blue (A–D). (A) The arrows indicate the blue stain characteristic for the reaction with ferrous compounds in the promastigote

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

• Qixiong Lin,
• Yueyou Peng,
• Yanyan Wen,
• Xiaoqiong Li,
• Donglian Du,
• Weibin Dai,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2023, 14, 262–279, doi:10.3762/bjnano.14.24

• the ability to target tumor tissue and enable multimodal imaging was fabricated for more efficient diagnosis and treatment by the Shu group. The biomimetic nanoformulation utilized hollow mesoporous Prussian blue nanoparticles as the core, endowing it with photothermal conversion and photoacoustic

Facile preparation of Au- and BODIPY-grafted lipid nanoparticles for synergized photothermal therapy

• Yuran Wang,
• Xudong Li,
• Haijun Chen and
• Yu Gao

Beilstein J. Nanotechnol. 2022, 13, 1432–1444, doi:10.3762/bjnano.13.118

• the complex pathogenic mechanisms of cancer, synergistic PTT with two or more PTAs that suppress tumor cells via different pathways is an appealing strategy to treat cancer. Yu et al. coated prussian blue (PB) on NaNdF4 nanoparticles to fabricate core–shell nanocomplexes with improved photothermal

Recent advances in nanoarchitectures of monocrystalline coordination polymers through confined assembly

• Lingling Xia,
• Qinyue Wang and
• Ming Hu

Beilstein J. Nanotechnol. 2022, 13, 763–777, doi:10.3762/bjnano.13.67

• to form monocrystalline coordination polymers embedding a fast electron transfer route [110]. The mixed ion-electron of Prussian blue crystals could be significantly enhanced under low temperature (i.e., −20 °C), which is important for the use of batteries in cold regions. To encapsulate conductive

• networks into Prussian blue single crystals, a gradient crystallization environment was built up through using a volatile inhibiting agent [111]. Evaporation of the inhibiting agent naturally forms a vertical gradient in the reactor, forcing nucleation and growth of single crystals inside the network at

• increased by using these materials as positive electrodes. The networks can also help to accelerate ion transfer in coordination polymers. When the Prussian blue nanocrystals contain a double-network PAAm/PAMPS hydrogel, the uptake of Cs+ ions from solution could be as high as 397 mg·g−1, which is very

Atomic layer deposited films of Al₂O₃ on fluorine-doped tin oxide electrodes: stability and barrier properties

• Hana Krýsová,
• Michael Neumann-Spallart,
• Hana Tarábková,
• Pavel Janda,
• Ladislav Kavan and
• Josef Krýsa

Beilstein J. Nanotechnol. 2021, 12, 24–34, doi:10.3762/bjnano.12.2

• dissolution rate of approx. 1 nm/h was estimated. The voltammogram of bare FTO (Figure 6, black curve) exibited additional waves near 0.8–0.9 V assigned to a Prussian blue deposit, which is known to sometimes interfere with the blocking tests with ferrocyanide/ferricyanide [23]. Interestingly, a clean surface

• (free of Prussian blue) was observed when the Al2O3-protected electrode was denuded by the dissolution of its coating, both in acidic (Figure 6) and alkaline (Figure 4) media. Exposure to phosphate buffer (pH 7.2) Cyclic voltammetry curves of 17 nm thick Al2O3 films on FTO substrates (in the presence of

Self-standing heterostructured NiC_x-NiFe-NC/biochar as a highly efficient cathode for lithium–oxygen batteries

• Shengyu Jing,
• Xu Gong,
• Shan Ji,
• Linhui Jia,
• Bruno G. Pollet,
• Sheng Yan and
• Huagen Liang

Beilstein J. Nanotechnol. 2020, 11, 1809–1821, doi:10.3762/bjnano.11.163

• catalysts is highly desirable for practical applications in lithium–oxygen batteries. Herein, a heterostructure of NiFe and NiCx inside of N-doped carbon (NiCx-NiFe-NC) derived from bimetallic Prussian blue supported on biochar was developed as a novel self-standing cathode for lithium–oxygen batteries. The

• develop low-cost FeC@N-C catalysts with high catalytic performance is desirable to replace the conventional PGM-based ORR and OER catalysts. Bimetallic Prussian blue analogues (PBAs) are metal organic framework (MOF) materials, which are promising precursors to prepare transition metal carbides with a

• the previous literatures [40][41][42]. However, a hydrothermal process was introduced here as a new treatment step prior to the precursor calcination in order to modify the properties of the prepared electrode materials. Heterostructured NiCx-NiFe-NC derived from bimetallic Prussian blue supported on

Photothermally active nanoparticles as a promising tool for eliminating bacteria and biofilms

• Mykola Borzenkov,
• Piersandro Pallavicini,
• Angelo Taglietti,
• Laura D’Alfonso,
• Maddalena Collini and
• Giuseppe Chirico

Beilstein J. Nanotechnol. 2020, 11, 1134–1146, doi:10.3762/bjnano.11.98

• materials, nanoscale metal chalcogenides (Cu2−xE, E = S, Se, Te), transition metal dichalcogenide nanostructures (e.g., WS2, MoS2), metal-oxide nanoparticles (e.g., WO3), and nanoscale coordination compounds (e.g., Prussian blue nanoparticles) [33][36][37][38]. The photothermal properties of these

• of Gram-positive and Gram-negative bacteria using the PVP-coated Prussian blue nanoparticles was one of the first experiments performed within the topic [73]. The photothermal effect was investigated at 810 nm and 980 nm under a 1 W·cm−2 irradiance. By choosing a proper nanoparticle concentration

• , the bacteria was selectively eliminated from the HeLa cells. In a later study, published in 2017, the photothermally active Prussian blue nanoparticles were grafted on a glass surface via a layer-by-layer approach [74]. The self-assembled monolayers demonstrated photothermally induced antibacterial

Uniform Fe₃O₄/Gd₂O₃-DHCA nanocubes for dual-mode magnetic resonance imaging

• Miao Qin,
• Yueyou Peng,
• Mengjie Xu,
• Hui Yan,
• Yizhu Cheng,
• Xiumei Zhang,
• Di Huang,
• Weiyi Chen and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2020, 11, 1000–1009, doi:10.3762/bjnano.11.84

• maintained an oversaturated state and kept generating the T2 signals since its concentration was higher than Gd2O3 in the nanocubes. Prussian blue staining further confirmed the presence of iron in lumbar muscles, indicating that the T1 and T2 signal changes were indeed induced by the FGDA nanocubes (Figure

• % paraformaldehyde solution and processed for Prussian blue staining. In vivo toxicity evaluation of FGDA nanocubes FGDA nanocubes were injected intravenously at a dose of 2 mg Fe/kg. Alternatively, the control group received a normal saline intravenous injection. After two weeks, the rats were euthanized by

• nanocubes. (b) T1 and (c) T2 SNR change post-intravenous injection of FGDA nanocubes. (d) Prussian blue staining in the lumbar muscle of a rat that received an intravenous injection of FGDA nanocubes. Scale bar: 100 μm. (e) H&E staining of heart, liver, spleen, lung and kidney. Top images: control group

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• Prussian blue (PB) interlayer and a gold shell. The enzymes horseradish peroxidase and glucose oxidase were immobilized to improve sensitivity, with linear ranges between 0.01 and 80.0 ng·mL−1 for CEA and from 0.014 to 142 ng·mL−1 for AFP, and detection limits of 4 pg·mL−1 and 7 pg·mL−1, respectively [25

Widening of the electroactivity potential range by composite formation – capacitive properties of TiO₂/BiVO₄/PEDOT:PSS electrodes in contact with an aqueous electrolyte

• Konrad Trzciński,
• Mariusz Szkoda,
• Andrzej P. Nowak,
• Marcin Łapiński and
• Anna Lisowska-Oleksiak

Beilstein J. Nanotechnol. 2019, 10, 483–493, doi:10.3762/bjnano.10.49

• –inorganic composites with TiO2 [18][19], organic–inorganic hybrids consisting of a conducting polymer and Prussian blue analogues [20], or composites with carbon nanomaterials [21]. Tuning of the electrochemical activity of supercapacitors can also be achieved via electrolyte modification. The addition of

Antitumor magnetic hyperthermia induced by RGD-functionalized Fe₃O₄ nanoparticles, in an experimental model of colorectal liver metastases

• Oihane K. Arriortua,
• Eneko Garaio,
• Borja Herrero de la Parte,
• Maite Insausti,
• Luis Lezama,
• Fernando Plazaola,
• Jose Angel García,
• Jesús M. Aizpurua,
• Maialen Sagartzazu,
• Mireia Irazola,
• Nestor Etxebarria,
• Ignacio García-Alonso,
• Alberto Saiz-López and
• José Javier Echevarria-Uraga

Beilstein J. Nanotechnol. 2016, 7, 1532–1542, doi:10.3762/bjnano.7.147

• Perls’ Prussian Blue stain concluded that deposits of iron could be observed within hepatic tissue, as blue dots corresponding to iron phagocytosis by Kupffer cells. On the other hand, when observing the tumor tissue, iron was seen as scattered deposits within the peripheral fibro-vascular matrix

• wet tissue was assessed in frozen samples by ICP–MS. From samples embedded in paraffin, sections were obtained and stained with hematoxylin-eosin and Perls’ Prussian Blue. During tumor growth, areas of tissue necrosis appear within the implants, and their size directly correlates with tumor volume

• with Prussian Blue. Statistical analysis Quantitative variables are described by their mean and standard deviation (SD) or by their median and range. Statistical analysis was carried out using nonparametric and nonpaired tests: Mann Whitney U test. For two-variable data sets, we produced box plots

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• their cellular uptake, the mechanism of internalization, cytotoxicity, viability and proliferation of neural stem cells, and compared them to the commercially available dextran-coated nanomag®-D-spio nanoparticles. Results: Light microscopy of Prussian blue staining revealed a concentration-dependent

• than that of nanomag®-D-spio To evaluate the uptake of nanoparticles by NSCs, Prussian blue staining was used. Both types of nanoparticles were taken up by the NSCs depending on concentration (Figure 2). When the same concentration of nanoparticles (0.2 mg/mL) was used, PLL-γ-Fe2O3-labeled cells were

• more intensely stained with Prussian blue than those labeled by nanomag®-D-spio. Considerably higher concentrations of nanomag®-D-spio (4.0 mg/mL) than PLL-γ-Fe2O3 (0.02 mg/mL) were needed for similar NSC cytoplasmic labeling. To quantify the efficiency of cell labeling an acoustic focusing cytometer

Molecular materials – towards quantum properties

• Mario Ruben

Beilstein J. Nanotechnol. 2015, 6, 1485–1486, doi:10.3762/bjnano.6.153

• or Prussian blue nano-arrays. Complementary, quantum chemical calculations have addressed lanthanide complexes and metal-organic frameworks. This Thematic Series is part of a subsession of the same title, which took place at the E-MRS spring meeting in May 2014 in Lille, France. I would like to

Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

• Lisa Landgraf,
• Ines Müller,
• Peter Ernst,
• Miriam Schäfer,
• Christina Rosman,
• Isabel Schick,
• Oskar Köhler,
• Hartmut Oehring,
• Vladimir V. Breus,
• Thomas Basché,
• Carsten Sönnichsen,
• Wolfgang Tremel and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

• microscopic analysis of the cells. Three different field-of-views, with a surface area of 74 mm2 each, were randomly selected and the number of all visible cells was counted. Afterwards, fluorescence (FITC) or bright-field microscopy (Prussian blue) was completed to measure the presence of cells loaded with

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

• Anja Ostrowski,
• Daniel Nordmeyer,
• Alexander Boreham,
• Cornelia Holzhausen,
• Lars Mundhenk,
• Christina Graf,
• Martina C. Meinke,
• Annika Vogt,
• Sabrina Hadam,
• Jürgen Lademann,
• Eckart Rühl,
• Ulrike Alexiev and
• Achim D. Gruber

Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25

• special stains for iron (Figure 1b), including Turnbull blue and Prussian blue [45][48][50], which are usually used to label pigments containing biogenic iron, such as hemosiderin [51]. Alcian blue is a histologic stain for the detection of negatively charged sulfate groups that occur, for example, in

Hybrid spin-crossover nanostructures

• Carlos M. Quintero,
• Gautier Félix,
• Iurii Suleimanov,
• José Sánchez Costa,
• Gábor Molnár,
• Lionel Salmon,
• William Nicolazzi and
• Azzedine Bousseksou

Beilstein J. Nanotechnol. 2014, 5, 2230–2239, doi:10.3762/bjnano.5.232

• work revealed exciting applications for high density read/write optical memory devices based on SCO compounds. As far as we know, the second and third types of core–shell structures were only achieved thus far by using Prussian blue analog complexes (PBA). While not all of these compounds are

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• higher catalytic activity than bulk tungsten trioxide. Other materials, such as cobalt-Prussian blue nanoparticles [75], Zn–Al layered double hydroxide [76], hydroxyapatite [77], and calcium carbonate [78], were also prepared within, or in the presence of, chitosan gels. In a biological approach, calcium

Towards bottom-up nanopatterning of Prussian blue analogues

• Virgile Trannoy,
• Marco Faustini,
• David Grosso,
• Sandra Mazerat,
• François Brisset,
• Alexandre Dazzi and
• Anne Bleuzen

Beilstein J. Nanotechnol. 2014, 5, 1933–1943, doi:10.3762/bjnano.5.204

• monolayers fabricated through sol–gel chemistry were used to grow isolated particles of Prussian blue analogues (PBA). The elaboration of the TiO2/CoFe PBA nanocomposites involves five steps. The samples were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), infrared

• . Keywords: nanopatterning; nanoperforated oxide monolayer; Prussian blue analogues; Introduction The development of methods to place nanoparticles into spatially well-defined, ordered arrays is one challenging aspect of nanotechnology. This is usually achieved by using top-down approaches, implementing

• to sol–gel chemistry combined with organic templating agents. Coordination chemistry allowing for the controlled assembly of a large variety of transition metal building units is preferred to build the functional compound. Prussian blue analogs (PBAs) are interesting for the design of bistable

Ultramicrosensors based on transition metal hexacyanoferrates for scanning electrochemical microscopy

• Maria A. Komkova,
• Angelika Holzinger,
• Andreas Hartmann,
• Alexei R. Khokhlov,
• Christine Kranz,
• Arkady A. Karyakin and
• Oleg G. Voronin

Beilstein J. Nanotechnol. 2013, 4, 649–654, doi:10.3762/bjnano.4.72

• electrochemical deposition of six layers of hexacyanoferrates (HCF), more specifically, an alternating pattern of three layers of Prussian Blue and three layers of Ni–HCF. The microelectrodes modified with mixed layers were continuously monitored in 1 mM hydrogen peroxide and proved to be stable for more than 5 h

• under these conditions. The mixed layer microelectrodes exhibited a stability which is five times as high as the stability of conventional Prussian Blue-modified UMEs. The sensitivity of the mixed layer sensor was 0.32 A·M−1·cm−2, and the detection limit was 10 µM. The mixed layer-based UMEs were used

• as sensors in scanning electrochemical microscopy (SECM) experiments for imaging of hydrogen peroxide evolution. Keywords: energy related; hydrogen peroxide; nanomaterials; nickel hexacyanoferrate; Prussian Blue; scanning electrochemical microscopy; ultramicroelectrodes; Introduction The detection

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• removed and 0 to 320 µg/mL of SN38-loaded Fe/Fe3O4 nanoparticles in fresh medium was added. After 24 h, the medium was removed; the cells were washed with 1× PBS three times, and stained with Prussian blue and counter stained by nuclear fast red to confirm that the loaded nanoparticles were iron/iron

• interested in the long term toxicity without activating the prodrug and changing cell morphology after loading. We have found that these nanoparticles showed no further toxicity even after five days (Figure 5). The successful loading of MNP-SN38 was confirmed by Prussian blue staining [53]. Nanoparticle

• : Prussian blue staining and counter stained by nuclear fast red 20×; b: 40×; c: control double-stable Mo/Ma Prussian blue stained and counter stained by nuclear fast red 20× (all images were taken in bright field). Flow cytometry of MNP-SN38 loaded double-stable Mo/Ma after 24 h. Side scatter was used to